Contents: Doctor Octopus, Electro, Hobgoblin, Kraven the Hunter, Scorpion, and Vulture. The Sinister Six, Guerrila tactics, and Standard encounter sets.

Setup: Choose X villains at random, where X is 1 more than the number of players. Put those villains into play, place the active counter on the villain with the lowest activation order value, and set the other villains aside. Put the Light at the End side scheme into play, trap! side faceup.

Ambush! — Special: Choose a set-aside villain at random, put that villain into play, and place the active counter on it. (In expert mode, place 2 threat on Light at the End).

Forced Interrupt: When a villain would activate, if no villain is in play, resolve this card's "Ambush!" ability. Continue that activation.